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2 edition of Biochemical studies on biliary epithelial cells isolated from rat liver. found in the catalog.

Biochemical studies on biliary epithelial cells isolated from rat liver.

Maurizio Parola

Biochemical studies on biliary epithelial cells isolated from rat liver.

by Maurizio Parola

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Published by Brunel University in Uxbridge .
Written in English


Edition Notes

ContributionsBrunel University. Department of Biochemistry.
The Physical Object
Pagination249p. :
Number of Pages249
ID Numbers
Open LibraryOL14472287M

At one time it was thought that biliary epithelial cells simply formed the lining to the tubular conduits which constitute the biliary tract. Development of in vitro systems for culturing biliary epithelial cells has enabled functional studies which increasingly show that this is far from true, and that biliary epithelial cells do have important functional roles. After 6 hours of normothermic ex situ liver culture, morphology and DNA replication in hepatocytes and biliary epithelial cells were assessed and compared between the two groups.

Biochemical studies on biliary epithelial cells isolated from rat liver. Author: Parola, Maurizio ISNI: Awarding Body: Brunel University Current Institution: Brunel University Date of Award: Availability of Full Text. This could be an important limitation of this study, since recent studies suggest that hepatic stellate cells can act as progenitor cells in the injured liver. 34,35 By performing a lineage tracing based on HNF1β, a specific marker of cells from the biliary compartment, our study clearly demonstrates that during liver injury HNF1β + biliary.

It has recently been applied to the study of hepatic stellate cell activation. 23, 24, 25 This model appears especially well suited to the investigation of the effects of bile acids on biliary. Liver Epithelial/Oval Cell Lines. Liver epithelial cell lines are considered the cell culture counterpart of liver stem/progenitor cells (for a detailed review, see Grisham and Thorgeirsson.


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Biochemical studies on biliary epithelial cells isolated from rat liver by Maurizio Parola Download PDF EPUB FB2

laiinil of Hipalolosf, Vf, Eisevier Biochemical studies on bile duct epithelial cells isolated from rat liver Maurizio Parola', Kevin H. Cheeseman2, Maria E. Biocca', Mario U. Dianzani' by: Biochemical studies on bile duct epithelial cells isolated from rat liver.

Parola M(1), Cheeseman KH, Biocca ME, Dianzani MU, Slater TF. Author information: (1)Dipartimento di Medicina ed Oncologia Sperimentale, Universitá di Torino, Italy.

Erratum in J Hepatol Nov;11(3)Cited by: Maurizio Parola, Kevin H. Cheeseman, Maria E. Blocca, Mario U. Dianzani, Trevor F. Slater, Menadione and cumene hydroperoxide induced cytotoxicity in biliary epithelial cells isolated from rat liver, Biochemical Pharmacology, /(90), 39, 11, (), ().Cited by: ABSTRACT— A method has been developed for the isolation of a population of cells enriched in epithelial lining cells from the bile ducts of normal rats.

The procedure utilized digestion by pronase of the white strands of biliary and connective tissue which remained after hepatocytes had been mechanically removed from collagenase‐perfused by:   Bile ductular epithelial cells were isolated from rat liver after ligation of the common bile duct for 6 to 8 wk.

Bile ductular epithelial cells were essentially free (greater than 99%) of. Kumar U, Jordan TW. Isolation and culture of biliary epithelial cells from the biliary tract fraction of normal rats. Liver. Dec; 6 (6)– Yang L, Faris RA, Hixson DC.

Long-term culture and characteristics of normal rat liver bile duct epithelial cells. Gastroenterology. Mar; (3)– Ishii M, Vroman B, LaRusso NF. Understanding cellular sources of hepatocyte regeneration is critical for developing effective therapies for chronic liver diseases.

Xie and colleagues show that severe liver injuries can, without genetic interventions, induce biliary epithelial cells to significantly contribute to hepatocyte regeneration through direct lineage conversion. First, hepatoblasts are induced to differentiate to biliary epithelial cells around the portal vein.

This step is promoted by TGFβ and Notch signals as well as a transcription factor, Sall4, whereas it is inhibited by two transcription factors, Tbx3 and Hex. Biliary epithelial cells then undergo tubular morphogenesis and form the biliary tree.

Background/Aims: Morphological and functional heterogeneity of intrahepatic bile duct epithelial cells has been suggested in situ and in isolated cholangiocytes.

The aim of this study was to evaluate if: (a) bile ducts, when isolated, maintain morphometric parameters similar to ducts in situ, (b) cellular organelles show heterogeneity in ducts of different size, and (c) some features permit.

Bile ductular epithelial cells were isolated from rat liver after ligation of the common bile duct for 6 to 8 wk.

Bile ductular epithelial cells were essentially free (> 99%) of hepatocytes and were histochemically positive (> 80%) for γ‐glutamyl transpeptidase activity. This number is sufficient for use in cell culture and the raising of an antiserum to a BEC surface marker to permit cell isolation by flow cytofluorometry.

REFERENCES Berry, M.N. and Friend, D.S. () High-yield preparation of isolated rat liver parenchymal cens. A biochemical and fine structural study. Journal of Cell Biology, 43,   In the present study, therefore, we compared steady-state levels of specific Na+,H+-antiporter (NHE1) mRNA in hepatocytes and biliary epithelial cells isolated from normal rat liver, and Na'/H+-antiporter mRNA in biliary epithelial cells explored the expression of other Na+,H+-antiporter isoforms (NHE2, NHE3, NHE4) in both cell types.

Biliary epithelial cells (BEC), or cholangiocytes, line a complex tree-like 3-dimensional network of conduits within the liver that form the biliary tract. The biliary tree can be divided into extrahepatic and intrahepatic components, and receives its blood supply exclusively from hepatic artery branches (Fig.

) [1]. The ability to identify, isolate, and transplant progenitor cells from solid tissues would greatly facilitate the treatment of diseases currently requiring whole organ transplantation. In this study, cell fractions enriched in candidate epithelial progenitor cells from the rat pancreas were isolated and transplanted into the liver of an inbred strain of Fischer rats.

The aim of the present study was to develop a novel model by which to study tumor-stroma interactions using primary cultures of human biliary epithelial cells (hBECs) and stromal cells (SCs) in CCA. hBECs and SCs, isolated from surgical resections (n=10), were semi-purified by centrifugation on a Percoll gradient; hBECs were further.

Our study demonstrates that after 30 minutes warm ischemia oxygen supply during liver isolation is required for biliary epithelial cell proliferation.

Biliary epithelial cells in the livers isolated after completing 30 min of warm ischemia following 30 min cold perfusion with a sufficient oxygen supply were able to proliferate in ESLC.

Introduction Biliary Function. The primary function of cholangiocytes is to modify bile of canalicular origin before it reaches the duodenum through a series of secretory and absorptive events [, ].One of the most important and well-studied functions of cholangiocytes is secretin-induced release of bicarbonate into the bile [].Under physiological conditions, bile secretion.

The outcome of liver injury is dictated by the effectiveness of repair. Successful repair (i.e., regeneration) results in replacement of dead epithelial cells with healthy epithelial cells, and reconstructs normal hepatic structure and regeneration is known to involve replication of surviving mature hepatocytes and bile duct cells.

Abstract. The idea that rodent liver contains stem-like * epithelial cells that can give rise to hepatocytes and biliary epithelial cells has developed from evidence that has accumulated from nearly years of investigation into the biology of the liver.

Early in this century, investigators recognized potential lineage relationships among biliary epithelial cells, transitional cells and.

Primary biliary cirrhosis (PBC) is characterized by a highly selective autoimmune injury of intrahepatic bile ducts. 1 Evidence suggests that there is a direct role of biliary epithelial cells (BECs) in the T cell–mediated insult through features of BECs that render such cells unique targets.

2 However, the recurrence of PBC following liver transplantation 3 militates against this thesis. Abstract. The establishment of liver epithelial cell lines or strains from newborn or adult rat liver has been reported by many investigators ().The cells of these lines are smaller in size and morphologically more simple than hepatocytes, have a considerable growth potential, and are easy to passage.the developmental potential of fetal rat liver cells toward the biliary epithelial cell lineage, the in vitro assay was performed using cells isolated from livers of E18 rats and also from 2-day-old (1*2)and P14 rats.

While a slight expression of BDS- was induced in cell culture from. Single-cell RNA sequencing of cells from healthy human liver, hepatocellular carcinoma and chimaeric mouse liver identifies subtypes of liver cells, epithelial .